Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].

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Gross findings in rams experimentally infected with Actinobacillus seminis. These results suggest that the kDa protein could be specific to A. In most chronic cases, testicular consistency tends to increase, reflecting the atrophy and fibrosis, independent of involved etiologic agent [ 313 ].

Identification of an immunogenic protein of Actinobacillus seminis that is present in microvesicles

The frequency of positive rams by bacteriology over the experimental period is presented in Figure 3. The vesicle diameter varied from 20 to nm, as was described for A.

Unfortunately this was not practical in this case. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Differences were considered statistically different when. In all the figures, lane 1 consists of molecular mass markers. Then ductal rupture can occur with extravasation of sperm, followed by diffuse fibrosis and granulomatous inflammation and formation of spermatic granulomas, as reported in this and other studies [ 81216 ].


The polypeptide with a molecular mass of 75 kDa identified in the fractions and had an apparently specific immunogenic reaction: Based on these results, we concluded that A. A previous study has shown that rams inoculated with A. These changes were observed in Results Prior to inoculation, all 20 rams were clinically healthy, without palpable lesions in the testes or epididymides.

For differential diagnosis, it is important to use diagnostic techniques for direct confirmation of the etiologic agent. The main lesions observed were an increase in volume and abscesses in the tail of the left epididymis Figure 6 a.

Immunohistochemical technique used was only effective for immunolabeling A. View at Google Scholar S. Anim Rev Vet Microbiol. However, none of the two agents demonstrated evidence of hematogenous dissemination, since they were not isolated from liver and spleen samples, and bacteremia was actinobaci,lus detected at any time point during the course of experimental infections.

There is a single report of A. Tsai C, Frasch CE. The length was measured in dorsoventral and width in craniocaudal direction. Subclinical cases have been known to persist for up to 4 years. Am J Vet Res.


For the determination of tissue distribution of agents, fragments of tail, body and head of the epididymes, testicles, ampullae of the vas deferens, seminal vesicles, bulbourethral glands, kidneys, bladder, inguinal and iliac lymph nodes, spleen, and liver were collected.


Measurements of the right tail of the epididymides remained unchanged throughout the course of the experimental infections Figure 2. Group 1 consisted of A. The Scientific World Journal.

To the best of our knowledge, this is the 1st report of data on the proteins in subcellular fractions of A. The place was decontaminated with broom fire and utensils used to feed, handle, or collect biological samples of the animals were decontaminated by sterilization or with hypochlorite solution prior to use in other group.

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The most severe microscopic lesions were observed at the site of inoculation i. A sparse predominant growth of gram-negative rods was obtained from the liver, and as part of a moderate mixed growth from abomasal contents. Ovine epididymitis caused by Actinobacillus seminis.